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File009 - Structural biology of human brain CNP, a protein essential for axonal survival2005-022007-04

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools
DocumentFinal report - Structural biology of human brain CNP, a protein essential for axonal survival2007

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.01
Location: Arquivo PCA - Pasta 8/2004
Title:
Final report - Structural biology of human brain CNP, a protein essential for axonal survival
Publication year: 2007
URL:
http://www.bial.com/imagem/Bolsa0904_03122012.pdf
Abstract/Results: RESULTS:
Our study was originally concerned with full-length brain Cyclic Nucleotide Phosphodiesterase (CNP), a member of the 2H phosphodiesterase family, which is found abundantly in the myelin of oligodendrocytes in the central nervous system, and has been shown to be of major importance for axonal survival. As such, CNP is believed to be implicated in diseases such as multiple sclerosis and schizophrenia. The three-dimensional structure of the full-length protein is anticipated to yield new insights into the molecular mechanisms of the protein.
We have developed a protocol for expression and purification of the recombinant full-length protein, verified the integrity of the protein by mass spectrometry, and checked the fold and its stability by circular dichroism and fluorescence spectroscopy. Extensive crystallisation trials did not yield crystals suitable for X-ray diffraction so far. In selected trials, protein self-organisation was observed, probably indicating epitaxy-like formation of nanostructures on the glass plates.
Another family of brain proteins are the Visinin-like Proteins (VILIPs) that are neuronal calcium sensor proteins and an important factor for synaptic plasticity. They are involved in schizophrenia, neurodegenerative diseases such as Alzheimer’s and other disorders.
To obtain further insights into the molecular level mechanisms of VILIPs, homology models were generated and modelling techniques were used to elucidate the binding mechanisms of these proteins to specific membrane components (PIPs). We have developed the first three-dimensional model of VILIP:PIP complexesm, and propose a mechanism whereby recognition of specific PIP derivatives contributes to the targeting of VILIPs to subcellular locations.
Accessibility: Document exists in file
Copyright/Reproduction:
By permission
Language:
eng
Author:
Hofmann, A.
Secondary author(s):
Zhou, L., Kämäräinen, O.
Document type:
Final report
Number of reproductions:
1
Indexed document:
No
Keywords: Psychophysiology / Psychopathology / Visinin-like proteins / Neuronal calcium sensors

Final report - Structural biology of human brain CNP, a protein essential for axonal survival

Final report - Structural biology of human brain CNP, a protein essential for axonal survival
DocumentDivalent cations and redox conditions regulate the molecular structure and function of Visinin-like Protein 12011

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.02
Location: Arquivo PCA - Pasta 8/2004
Title:
Divalent cations and redox conditions regulate the molecular structure and function of Visinin-like Protein 1
Publication year: 2011
URL:
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0026793&representation=PDF
Abstract/Results: ABSTRACT:
The NCS protein Visinin-like Protein 1 (VILIP-1) transduces calcium signals in the brain and serves as an effector of the non-retinal receptor guanylyl cyclases (GCs) GC-A and GC-B, and nicotinic acetyl choline receptors (nAchR). Analysis of the quaternary structure of VILIP-1 in solution reveals the existence of monomeric and dimeric species, the relative contents of which are affected but not exclusively regulated by divalent metal ions and Redox conditions. Using small-angle X-ray scattering, we have investigated the low resolution structure of the calcium-bound VILIP-1 dimer under reducing conditions. Scattering profiles for samples with high monomeric and dimeric contents have been obtained. The dimerization interface involves residues from EF-hand regions EF3 and EF4.Using monolayer adsorption experiments, we show that myristoylated and unmyristoylated VILIP-1 can bind lipid membranes. The presence of calcium only marginally improves binding of the protein to the monolayer, suggesting that charged residues at the protein surface may play a role in the binding process.In the presence of calcium, VILIP-1 undergoes a conformational re-arrangement, exposing previously hidden surfaces for interaction with protein partners. We hypothesise a working model where dimeric VILIP-1 interacts with the membrane where it binds membrane-bound receptors in a calcium-dependent manner.
Accessibility: Document does not exist in file
Language:
eng
Author:
Wang, C. K.
Secondary author(s):
Simon, A., Jessen, C. M., Oliveira, C. L., Mack, L., Braunewell, K.-H., Ames, J. B., Pedersen, J. S., Hofmann, A.
Document type:
Article
Number of reproductions:
1
Reference:
Wang, C. K., Simon, A., Jessen, C. M., Oliveira, C. L., Mack, L., Braunewell, K.-H., . . . Hofmann, A. (2011). Divalent cations and redox conditions regulate the molecular structure and function of Visinin-like Protein 1. PLoS ONE, 6(11), e26793. https://doi.org/10.1371/journal.pone.0026793
2-year Impact Factor: 4.092|2011
Times cited: 12|2025-02-10
Indexed document: Yes
Quartile: Q1
Keywords: Psychophysiology / Guanylyl cyclase / Neuronal calcium sensors / Visinin-like proteins

Divalent cations and redox conditions regulate the molecular structure and function of Visinin-like Protein 1

Divalent cations and redox conditions regulate the molecular structure and function of Visinin-like Protein 1
DocumentVILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons2006

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.03
Location: Arquivo PCA - Pasta 8/2004
Title:
VILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons
Publication year: 2006
URL:
http://www.researchgate.net/publication/29470956_VILIP-1_A_Novel_Regulator_of_the_Guanylate_Cyclase_Transduction_System_in_Neurons
Abstract/Results: ABSTRACT:
VILIP-1 belongs to the subfamily of Visinin-like proteins with its members VILIP-1, -2, -3, hippocalcin and neurocalcin d which have been implicated in the modulation of a variety of intracellular signaling cascades. VILIP-1 has recently been characterized in more detail and an involvement in cyclic nucleotide signaling has been demonstrated. Thus, similar to other NCS proteins such as GCAPs and neurocalcin d, VILIP-1 can influence the activtiy of guanylyl cyclase enzymes and the cGMP-signaling system of neurons in the nervous
system.
Accessibility: Document does not exist in file
Language:
eng
Author:
Braunewell, K.-H.
Secondary author(s):
Brackmann, M., Hofmann, A.
Document type:
Article
Number of reproductions:
1
Reference:
Braunewell, K.-H., Brackmann, M., & Hofmann, A. (2006). VILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons. Calcium Binding Proteins, 1(1), 12-15.
Indexed document: No
Keywords: Calcium-myristoyl switch / Guanylyl cyclase / Intracellular signaling pathways / Neuronal calcium sensors / Visinin-like proteins

VILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons

VILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons
DocumentStructure, function and expression of members of the VILIP (visinin-like protein) subfamily of neuronal Ca2+ sensor proteins in Neuronal calcium sensor proteins2006

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.04
Location: Arquivo PCA - Pasta 8/2004
Title:
Structure, function and expression of members of the VILIP (visinin-like protein) subfamily of neuronal Ca2+ sensor proteins in Neuronal calcium sensor proteins
Publication year: 2006
URL:
http://books.google.pt/books?id=gT-mNaGTRhMC&pg=PA115&lpg=PA115&dq=Structure,+function+and+expression+of+members+of+the+VILIP+(visinin-like+protein)+subfamily+of+neuronal+Ca2%2B+sensor+proteins+in+Neuronal+calcium+sensor+proteins&source=bl&ots=NJwxim7rp0&sig=CA37gZ1oRIypJ-Heff3z08OxSMw&hl=pt-PT&sa=X&ei=s_DsUZTGCqbX7Ab6yICoCA&ved=0CEwQ6AEwAw#v=onepage&q=Structure%2C%20function%20and%20expression%20of%20members%20of%20the%20VILIP%20(visinin-like%20protein)%20subfamily%20of%20neuronal%20Ca2%2B%20sensor%20proteins%20in%20Neuronal%20calcium%20sensor%20proteins&f=false
Accessibility: Document does not exist in file
Language:
eng
Author:
Brackmann, M.
Secondary author(s):
Hofmann, A., Braunewell, K.-H.
Document type:
Book chapter
Number of reproductions:
1
Reference:
Brackmann, M., Hofmann, A., & Braunewell, K.-H. (2006). Structure, function and expression of members of the VILIP (visinin-like protein) subfamily of neuronal Ca2+ sensor proteins . In P. Phillipov & K.-W. Koch (Eds.), Neuronal calcium sensor proteins (pp. 115-135). Hauppauge, NY: Nova Science Publishers.
Indexed document: No
Keywords: Adenylyl cyclase / Calcium-myristoyl switch / Guanylyl cyclase / Intracellular signaling pathways / MAPK pathways / Neuronal calcium sensors / Nicotinic acetylcholine receptor / Visinin-like proteins
DocumentStructural biology of human brain CNP, a protein essential for axonal survival2008

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.05
Location: Arquivo PCA - Pasta 8/2004
Title:
Structural biology of human brain CNP, a protein essential for axonal survival
Publication year: 2008
URL:
http://www.bial.com/imagem/Actas_7Simp.pdf
Abstract/Results: ABSTRACT:
OBJECTIVES:
Structure-function relationships of brain proteins need to be established in order to understand the molecular basis of brain disorders that affect organic health, as well as the psychological well-being.
METHODS:
Proteins have been prepared and purified, and subjected to structural investigation using spectroscopic and crystallographic methods. Molecular modelling has been used to assess protein-ligand binding.
RESULTS AND CONCLUSIONS:
Our study was originally concerned with full-length brain Cyclic Nucleotide Phosphodiesterase (CNP), a member of the 2H phosphodiesterase family, which is found abundantly in the myelin of oligodendrocytes in the central nervous system, and has been shown to be of major importance for axonal survival. As such, CNP is believed to be implicated in diseases such as multiple sclerosis and schizophrenia. The threedimensional structure of the full-length protein is anticipated to yield new insights into the molecular mechanisms of the protein. We have developed a protocol for expression and purification of the recombinant full-length protein, verified the integrity of the protein by mass spectrometry, and checked the fold and its stability by circular dichroism and fluorescence spectroscopy. Extensive crystallisation trials did not yield crystals suitable for X-ray diffraction so far. In selected trials, protein self-organisation was observed, probably indicating epitaxy-like formation of nanostructures on the glass plates. Another family of brain proteins are the Visinin-like Proteins (VILIPs) that are neuronal calcium sensor proteins and an important factor for synaptic plasticity. They are involved in schizophrenia, neurodegenerative diseases such as Alzheimer’s and other disorders.
To obtain further insights into the molecular level mechanisms of VILIPs, homology models were generated and modelling techniques were used to elucidate the binding mechanisms of these proteins to specific membrane components (PIPs). We have developed the first three-dimensional model of VILIP:PIP complexes, and propose a mechanism whereby recognition of specific PIP derivatives contributes to the targeting of VILIPs to subcellular locations.
PUBLICATIONS:
Braunewell, K.H., Altarche-Xifro, W., Lange, K. & Hofmann, A. (2007) Modelling of VILIPPhosphatidylinositol Interactions - Implications for Differential Membrane Localisation of NCS proteins. Submitted.; Braunewell, K.H., Brackmann, M. & Hofmann, A. (2006) VILIP-1, A novel regulator of the guanylate cyclase transduction system in neurons. Calcium Binding Proteins 1, 12-15.; Brackmann, M., Hofmann, A. & Braunewell, K.-H. (2006) Structure, function and expression of members of the VILIP (visinin-like protein) subfamily of neuronal Ca2+ sensor proteins in Neuronal calcium sensor proteins (K.-W. Koch, Phillipov, P.), Nova Science Publisher, pp 115-135.
Accessibility: Document does not exist in file
Language:
eng
Author:
Hofmann, A.
Document type:
Conference abstract
Number of reproductions:
1
Reference:
Hofmann, A. (2008). Structural biology of human brain CNP, a protein essential for axonal survival. In Aquém e além do cérebro. Behind and beyond the brain. Proceedings of the 7th Symposium of Fundação Bial. Porto: Fundação Bial.
Indexed document: No
Keywords: Cyclic nucleotide phosphodiesterase / Protein structure-function relationships / Visinin-like proteins

Structural biology of human brain CNP, a protein essential for axonal survival

Structural biology of human brain CNP, a protein essential for axonal survival
DocumentComparison of VILIP-1 and VILIP-3 binding to phospholipid monolayers2014

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.06
Location: Arquivo PCA - Pasta 8/2004
Title:
Comparison of VILIP-1 and VILIP-3 binding to phospholipid monolayers
Publication year: 2014
URL:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093948
Abstract/Results: ABSTRACT:
The neuronal calcium sensor proteins Visinin-like Proteins 1 (VILIP-1) and 3 (VILIP-3) are effectors of guanylyl cyclase and acetyl choline receptors, and transduce calcium signals in the brain. The "calcium-myristoyl" switch, which involves a post-translationally added myristoyl moiety and calcium binding, is thought to regulate their membrane binding capacity and therefore, play a critical role in their mechanism of action. In the present study, we investigated the effect of membrane composition and solvent conditions on the membrane binding mechanisms of both VILIPs using lipid monolayers at the air/buffer interface. Results based on comparison of the adsorption kinetics of the myristoylated and non-myristoylated proteins confirm the pivotal role of calcium and the exposed myristol moiety for sustaining the membrane-bound state of both VILIPs. However, we also observed binding of both VILIP proteins in the absence of calcium and/or myristoyl conjugation. We propose a two-stage membrane binding mechanism for VILIP-1 and VILIP-3 whereby the proteins are initially attracted to the membrane surface by electrostatic interactions and possibly by specific interactions with highly negatively charged lipids head groups. The extrusion of the conjugated myristoyl group, and the subsequent anchoring in the membrane constitutes the second stage of the binding mechanism, and ensures the sustained membrane-bound form of these proteins.
Accessibility: Document exists in file
Language:
eng
Author:
Rebaud, S.
Secondary author(s):
Simon, A., Wang, C. K., Mason, L., Blum, L., Hofmann, A., Girard-Egrot, A.
Document type:
Article
Number of reproductions:
1
Reference:
Rebaud, S., Simon, A., Wang, C. K., Mason, L., Blum, L., Hofmann, A., & Girard-Egrot, A. (2014). Comparison of VILIP-1 and VILIP-3 binding to phospholipid monolayers. PLoS ONE, 9(4): e93948. https://doi.org/10.1371/journal.pone.0093948
2-year Impact Factor: 3.234|2014
Times cited: 10|2025-02-10
Indexed document: Yes
Quartile: Q1

Comparison of VILIP-1 and VILIP-3 binding to phospholipid monolayers

Comparison of VILIP-1 and VILIP-3 binding to phospholipid monolayers
DocumentInteractions of visinin-like proteins with phospho-inositides2010

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.07
Location: Arquivo PCA - Pasta 8/2004
Title:
Interactions of visinin-like proteins with phospho-inositides
Publication year: 2010
URL:
https://www.publish.csiro.au/CH/CH09355
Abstract/Results: ABSTRACT:
The subcellular membrane localization of neuronal calcium sensor (NCS) proteins in living cells, such as Visinin-like Proteins-1 (VILIP-1) and VILIP-3, differs substantially. We have followed the hypothesis that the differential localization may be due to the specific binding capabilities of individual VILIPs for phosphatidylinositol phosphates (PIPs). Several highly conserved lysine residues in the N-terminal region could provide favourable electrostatic interactions. Molecular modelling results support a binding site for phospho-inositides in the N-terminal area of VILIP-1, and the involvement of the conserved N-terminal lysine residues in binding the phospho-inositol head group. Experimentally, the binding of VILIP-1 to inositol derivatives was tested by a PIP strip assay, which showed the requirement of phosphorylation of the inositol group for the interaction of the protein with PIPs. Monolayer adsorption measurements showed a preference of VILIP-1 binding to PI(4,5)P-2 over PI(3,4,5)P-3. The co-localization of VILIP-1 with PI(4,5)P-2 at the cell surface membrane in hippocampal neurons further supports the idea of direct interactions of VILIP-1 with PIPs in living cells.
Accessibility: Document does not exist in file
Language:
eng
Author:
Braunewell, K. H.
Secondary author(s):
Paul, B., Altarche-Xifro, W., Noack, C., Lange, K., Hofmann, A.
Document type:
Article
Number of reproductions:
1
Reference:
Braunewell, K. H., Paul, B., Altarche-Xifro, W., Noack, C., Lange, K., & Hofmann, A. (2010). Interactions of visinin-like proteins with phospho-inositides. Australian Journal of Chemistry, 63(3), 350-356. https://doi.org/10.1071/CH09355
2-year Impact Factor: 1.681|2010
Times cited: 10|2025-02-07
Indexed document: Yes
Quartile: Q2
DocumentSpecific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins2014

Reference code: PT/FB
Entity holding: BIAL Foundation
Location: S. Mamede do Coronado
Title:
BIAL Foundation Archive
Start date: 1994
History:
The BIAL Foundation was created in 1994 by Laboratórios BIAL in conjunction with the Council of Rectors of Portuguese Universities. BIAL’s Foundation mission is to foster the scientific study of Man from both the physical and spiritual perspectives.
Along the years the BIAL Foundation has developed an important relationship with the scientific community, first in Portugal and after worldwide. Today it is an institution of reference which aims to stimulate new researches that may help people, promote more health and contribute to new milestones to gain access to knowledge.
Among its activities the BIAL Foundation manages the BIAL Award, created in 1984, one of the most important awards in the Health field in Europe. The BIAL Award rewards both the basic and the clinical research distinguishing works of major impact in medical research.
The BIAL Foundation also assigns Scientific Research Scholarships for the study of neurophysiological and mental health in people, arousing the interest of researchers in the areas of Psychophysiology and Parapsychology.
To date the BIAL Foundation has supported 461 projects, more than 1000 researchers, with research groups in twenty-seven countries, resulting, until April 2013, in about 600 full papers, out of which 172 published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Since 1996 the BIAL Foundation organizes the Symposia entitled "Behind and Beyond the Brain", a Forum that gathers well renowned neurosciences speakers and the BIAL Foundation Fellows which are spread around the world.
Classified as an institution of public utility, the BIAL Foundation includes among its patrons the Portuguese President, the Portuguese Universities Rectors' Council and the Portuguese Medical Association.
URL: http://www.bial.com/pt/
Accessibility: By permission

Reference code: PT/FB/BL
Entity holding: BIAL Foundation
Title: BIAL Grants
Start date: 1994
History:
In 1994 the BIAL Foundation launched a programme of science research grants with the aim of encouraging the research into Man’s physical and mental processes, namely in fields still largely unexplored but which warrant further scientific analysis, as Psychophysiology and Parapsychology.
Since its launch, applications to the BIAL grants have been increasing. Up to now 461 projects have been supported, involving more than 1000 researchers from 27 countries.
The approved applications have benefited from grants in amounts comprised between €5,000 and €50, 000. The amount to be granted is fixed by the Scientific board according to the needs of each project.
The supported projects have originated, until April 2013, in about 600 full papers, 172 out of which were published in indexed international journals with an average impact factor of 3.6 and a substantial number of citations (1665).
Among the BIAL Foundation fellows is worth highlighting the presence of scientists from prestigious universities from the United States, United Kingdom, Australia, Russia, Germany, Japan, France, Canada, and many others.
The BIAL grants are promoted biannually.

Reference code: PT/FB/BL-2004
Location: Arquivo PCA - Pastas 1 a 25/2004
Title:
2004 Grants
Start date: 2005-01 - 2012-09
Dimension/support:
25 caixas de arquivo

Reference code: PT/FB/BL-2004-009
Location: Arquivo PCA - Pasta 8/2004
Title:
009 - Structural biology of human brain CNP, a protein essential for axonal survival
Duration: 2005-02 - 2007-04
Researcher(s):
Andreas Hofmann
Institution(s): Institute of Cell & Molecular Biology, The University of Edinburgh (UK)
Contents: Contents:
Bursary agreement
Application form
Correspondence
Financial report and expenditure documents
Progress report
Final report
Language: eng
Author:
Hofmann, A.
Number of reproductions:
1
Keywords:
Psychophysiology / Brain structure and function / Assessment tools

Reference code: PT/FB/BL-2004-009.08
Location: Arquivo PCA - Pasta 8/2004
Title:
Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins
Publication year: 2014
URL:
https://www.sciencedirect.com/science/article/pii/S0005273614002442?via%3Dihub
Abstract/Results: ABSTRACT:
VIsinin-LIke Proteins (VILIPs) are a subfamily of the Neuronal Calcium Sensor (NCS) proteins, which possess both N-myristoylation and EF-hand motifs allowing for a putative 'calcium-myristoyl switch' regulation mechanism. It has previously been established that myristoyl conjugation increases the affinity of proteins for membranes, but, in many cases, a second feature such as a cluster of positively-charged residues is needed for stable membrane binding. The interaction of two members of this family, VILIP-1 and VILIP-3, with Langmuir monolayers as membrane models has been investigated in order to study the effects of both myristoylation and the highly basic region containing conserved poly-lysine residues on membrane association kinetics and binding properties. Results show that in the presence of calcium, N-myristoylation significantly increases the kinetic rate of VILIP adsorption to the membrane. Additionally, the proteins bind to negatively charged phospholipids independently of the conjugated myristate moiety. Besides the regulatory effect of calcium on the rate of binding presumably due to exposure of the myristoyl moiety ascribed to their putative 'calcium-myristoyl switch', VILIP-1 and -3 also engage specific interactions with biomimetic membranes containing phosphatidylinositol 4,5-bisphosphate (PIP2). The presence of PIP2 increases the membrane association rates of both VILIPs. Taken together, these results show the major kinetic role of N-myristoylation for membrane binding, and highlight the critical role of specific phosphoinositide interactions for membrane association of members of the VILIP family.
Accessibility: Document exists in file
Language:
eng
Author:
Rebaud, S.
Secondary author(s):
Wang, C. K., Sarkis, J., Mason, L., Simon, A., Blum, L. J., Hofmann, A., Girard-Egrot, A. P.
Document type:
Article
Number of reproductions:
1
Reference:
Rebaud, S., Wang, C. K., Sarkis, J., Mason, L., Simon, A., Blum, L. J., Hofmann, A., Girard-Egrot, A. P. (2014). Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins. Biochimica et Biophysica Acta, 1838(10), 2698-2707. https://doi.org/10.1016/j.bbamem.2014.06.021
2-year Impact Factor: 3.836|2014
Times cited: 13|2025-02-10
Indexed document: Yes
Quartile: Q1
Keywords: Visinin-LIke Proteins (VILIPs) / Neuronal Calcium Sensor (NCS) proteins / Calcium-myristoyl switch / Phosphoinositides / Langmuir monolayer / Protein-lipid interactions

Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins

Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins